转基因大动物常用载体频数分析及筛查策略

doi:10.11843/j.issn.0366-6964.2017.12.003

Abstract

Abstract:

The recombinant expression vector is vital in production of genetically modified animals, which includes complete exogenous gene expression cassette containing the target gene promoter, terminator and reporter gene. This paper briefly introduces the genetically modified animal production technology and counts the vector frequency commonly used in the production of genetically modified pigs, cattle and sheep during nearly 10 years. The statistical results show that promoter frequency of the genetically modified pigs from more to less are casein, CAG, CMV promoters, terminator frequency are rabbit β-globin poly A, casein poly A, SV40 poly A and BGH poly A; The promoter frequency in genetically modified sheep from more to less are casein, BLG and CMV promoters and terminator are casein poly A, BLG poly A, BGH poly A, SV40 poly A and rabbit β-globin poly A; The promoter frequency in genetically modified cattle from more to less are casein, CMV and human α-lactalbumin promoters and terminator are SV40 poly A, BGH poly A and casein poly A. Multiple detection and screening strategies aiming at the promoter and terminator are proposed according to the statistical results. This paper also analyses the possible problems in the future in genetically modified animal detection, this review will provide reference for regulatory perspective and detection of genetically modified animals.

CLC Number:

Q812

LIU Xiao-fei, WANG Qin, LI Xiao-lin, LIU Dan-dan, QIU Song-yin, LIN Xiang-mei. Frequency Analysis of Vectors Used in Genetically Modified Animals and Screening Detection Review[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(12): 2239-2248.

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References

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Frequency Analysis of Vectors Used in Genetically Modified Animals and Screening Detection Review

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